Generally, all hormones are tested in order to see if there is an imbalance..either under or over production of that sort which leads to certain abnormalities in the body. Steroid hormones involves assays or test which is only specific to a single hormone and other tests on the other hand would perform singly but measures other closely related hormones.
Urine, blood, and even saliva are specimen tested in steroid hormone analysis but since blood is normally used in clinical chemistry, preferred blood products for steroid hormone test is serum or plasma.
Immunoassay Techniques
It is a test that has a specific approach and techniques are particularly attractive for measurement of plasma levels since they can detect much lower quantities than other methods. Examples are Radioimmunoassay (RIA) and Enzyme immunoassay, both are used to measure specific steroid component.
Although immunoassay techniques had an advantage over other methods like ease and speed of use because of automation, they are not reliable when it comes to steroid analysis.Since various steroids have the same structure, specificity of antibodies are hard to detect and differentiate from one another. To avoid this, proper precautions should be taken.
Reference: Clinical Chemistry: A Fundamental Textbook by Donald F. Calbreath
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Urine, blood, and even saliva are specimen tested in steroid hormone analysis but since blood is normally used in clinical chemistry, preferred blood products for steroid hormone test is serum or plasma.
Immunoassay Techniques
It is a test that has a specific approach and techniques are particularly attractive for measurement of plasma levels since they can detect much lower quantities than other methods. Examples are Radioimmunoassay (RIA) and Enzyme immunoassay, both are used to measure specific steroid component.
Although immunoassay techniques had an advantage over other methods like ease and speed of use because of automation, they are not reliable when it comes to steroid analysis.Since various steroids have the same structure, specificity of antibodies are hard to detect and differentiate from one another. To avoid this, proper precautions should be taken.
Reference: Clinical Chemistry: A Fundamental Textbook by Donald F. Calbreath
photo credits:
pic1
pic2
As I've mentioned above, other hormones can be tested using saliva as specimen. Is is called "Saliva Testing" which tests hormones such as estradiol, progesterone, testosterone and cortisol which are all under steroid hormones.
Please follow this link to know more about this test:
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